terminator codon中文是什么意思
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用"terminator codon"造句"terminator codon"怎么读"terminator codon" in a sentence
中文翻译手机版
- 终止密码子
- 终止信号
- "terminator"中文翻译 n. 1.限定者[物];终止者[物]。 2.【化学】(链 ...
- "codon"中文翻译 n. 【生物学】(遗传)密码子。
- "terminator codon mutation" 中文翻译 : 终止密码突变
- "codon" 中文翻译 : n. 【生物学】(遗传)密码子。
- "terminator" 中文翻译 : n. 1.限定者[物];终止者[物]。 2.【化学】(链的)终止剂;【生物化学】终止符。 3.【天文学】(月、星表面上的)明暗界线。
- "the terminator" 中文翻译 : 魔鬼终结者1; 终结者/魔鬼终结者/未来战士; 终结者i; 终结者特别版; 自家制素描
- "amber codon" 中文翻译 : 琥珀密码子,uag三联体密码子
- "ambiguous codon" 中文翻译 : 双关密码子,意义含糊的密码子,多义密码子; 意义含糊的密码子
- "ambivalent codon" 中文翻译 : 相克性密码子
- "codegenerate codon" 中文翻译 : 互简并密码子,简并密码子; 简并密码子
- "codon bias" 中文翻译 : 密码偏爱,密码子偏倚; 密码子偏倚(性)
- "codon degeneracy" 中文翻译 : 密码简并
- "codon differences" 中文翻译 : 密码子差数
- "codon family" 中文翻译 : 密码子偏倚; 密码子组
- "codon type" 中文翻译 : 密码子型; 密码子组
- "codon usage" 中文翻译 : 密码子使用,密码子选择; 密码子型; 密码子选择
- "degenerate codon" 中文翻译 : 简并密码子
- "hypervariable codon" 中文翻译 : 超变密码子; 超可变密码子
- "initia codon" 中文翻译 : 起始密码子
- "initiation codon" 中文翻译 : 起始密码子
- "initiator codon" 中文翻译 : 起动信号起始密码子; 起始密码
- "minority codon" 中文翻译 : 稀少密码子,调谐密码子
- "missence codon" 中文翻译 : 误义密码子
- "missense codon" 中文翻译 : 误义密码子
- "modulating codon" 中文翻译 : 蝶密码子
例句与用法
- In this paper , first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv . the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector . after transforming e . coli dh5 a , ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr . presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc . comparing the aquired sequence of 3abc with that of reference strains , the homology is more than 99 percent . the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo . lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene , which happened to form a terminator codon behind 3ab gene , but it contained the complete open reading frame ( orf ) of 3ab gene . positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ) , bacteria were detected by sds - page and western blotting after properly treated . the results showed that the 3ab gene expressed successfully in e . coli and 33 . 5ku fusion protein can be recognized by the positive bovine serum of fmdv . the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
扩增产物连接到pgem - teasy载体中,转化大肠杆菌dh5菌株,筛选氨苄青霉素抗性菌落,提取质粒经酶切鉴定、 pcr分析以及确证性测序证明,所克隆的1500bp左右的片段含有完整的3abc基因,与国外参考序列相比,同源性在99以上。将重组质粒pgem - 3abc和表达载体ptriex - 4neo分别用sal和bgl与xho和bgl消化后,亚克隆3abc基因至原核表达载体ptriex - 4neo中,通过酶切鉴定、 pcr扩增以及序列分析,发现克隆到ptriex - 4neo载体上的片段于3abc基因708bp处出现了17bp的缺失,碰巧在3ab基因后形成一终止密码子,但3ab基因的阅读框架完整,选出含有3ab基因完整阅读框架的阳性克隆,用iptg诱导表达,收集菌液进行sds - page电泳、 westernblotting分析,结果表明, 3ab基因在大肠杆菌中成功表达,其表达产物为分子量33 . 5ku的融合蛋白,并能被口蹄疫病毒阳性血清识别。经薄层扫描分析,表达量占总蛋白量的26以上。
其他语种
相关词汇
terminators 中文, terminator 中文, degenerate codon 中文, assault terminator 中文, null terminator 中文, field terminator 中文, cable terminator 中文, terminator board 中文, terminator record 中文, terminator 3 中文, terminator 3 rise of the machines 中文, terminator armour 中文, terminator code 中文, terminator codon mutation 中文, terminator dongle 中文, terminator factor 中文, terminator group 中文, terminator group controller 中文,
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